plasmid pen ttgmirc2 (Addgene inc)
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plasmid pen ttgmirc2
Plasmid Pen Ttgmirc2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pen+ttgmirc2/pEN_TTGmiRc2+(Plasmid+%2325753)/pmc12402202-7-0-7
Average 93 stars, based on 8 article reviews
Plasmid Pen Ttgmirc2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pen+ttgmirc2/pEN_TTGmiRc2+(Plasmid+%2325753)/pmc12402202-7-0-7
Average 93 stars, based on 8 article reviews
plasmid pen ttgmirc2 - by Bioz Stars,
2026-09
93/100 stars
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Generated:Article Title: Neural crest-related NXPH1/α-NRXN signaling opposes neuroblastoma malignancy by inhibiting metastasis Article Snippet: The pLKO.1-TRC cloning vector (RRID: Addgene_10878) and the pLKO.1-sh-Ctl (RRID: Addgene_10879) were kindly provided by Marian Martínez-Balbás. .. Doxycycline-inducible miRshRNA-eGFP-expressing pSLIK-Neo lentiviral vectors were generated by gateway recombination between the TTRE-eGFP-miR-shRNA entry vector and the pSLIK destination vector ( ). Article Title: An in vivo transfection system for inducible gene expression and gene silencing in murine hepatocytes Article Snippet: The entry vector for inducible gene expression of YAP-Flag was generated by amplification from p2xFlag CMV2-YAP2 (Addgene #19045) using touchdown PCR followed by subcloning into pEN_TTmcs (Addgene #25755). .. The entry vector for inducible shRNA expression was generated using a validated shRNA sequence against mouse Hnf4α 22 that was modified in accordance with the pSLIK cloning protocol 19 followed by subcloning into Article Title: Neural crest-related NXPH1/α-NRXN signaling opposes neuroblastoma malignancy by inhibiting organotropic metastasis. Article Snippet: Neuroblastoma is a pediatric cancer that can present as lowor high-risk tumors (LR-NBs and HR-NBs), the latter group showing poor prognosis due to metastasis and strong resistance to current therapy.. Whether LR-NBs and HR-NBs differ in the way they exploit the transcriptional program underlying their neural crest, sympatho-adrenal origin remains unclear.. Here, we identified the transcriptional signature distinguishing LR-NBs from HR-NBs, which consists mainly of genes that belong to the core sympathoadrenal developmental program and are associated with favorable patient prognosis and with diminished disease progression. Plasmid Preparation:Article Title: An in vivo transfection system for inducible gene expression and gene silencing in murine hepatocytes Article Snippet: The entry vector for inducible gene expression of YAP-Flag was generated by amplification from p2xFlag CMV2-YAP2 (Addgene #19045) using touchdown PCR followed by subcloning into pEN_TTmcs (Addgene #25755). .. The entry vector for inducible shRNA expression was generated using a validated shRNA sequence against mouse Hnf4α 22 that was modified in accordance with the pSLIK cloning protocol 19 followed by subcloning into Article Title: The Synthesis and Secretion of Versican Isoform V3 by Mammalian Cells: A Role for N-linked Glycosylation Article Snippet: .. Plasmid construction The open reading frame of rat V3 ( {"type":"entrez-nucleotide","attrs":{"text":"NM_001170559.1","term_id":"281604091","term_text":"NM_001170559.1"}} NM_001170559.1 ) was PCR-amplified with primers rV3SpeIF and rV3XbaIR ( ) introducing SpeI and XbaI restriction sites and a c-terminal Hag-tag and cloned into Article Title: Recombinant versican isoforms and related compositions and methods Article Snippet: .. Experimental Procedures Plasmid Construction The open reading frame of rat V3 (NM_001170559.1) was PCR-amplified with primers rV3SpeIF and rV3XbaIR (Table 3) introducing SpeI and XbaI restriction sites and a c-terminal hemagglutinin tag and cloned into shRNA:Article Title: An in vivo transfection system for inducible gene expression and gene silencing in murine hepatocytes Article Snippet: The entry vector for inducible gene expression of YAP-Flag was generated by amplification from p2xFlag CMV2-YAP2 (Addgene #19045) using touchdown PCR followed by subcloning into pEN_TTmcs (Addgene #25755). .. The entry vector for inducible shRNA expression was generated using a validated shRNA sequence against mouse Hnf4α 22 that was modified in accordance with the pSLIK cloning protocol 19 followed by subcloning into Expressing:Article Title: An in vivo transfection system for inducible gene expression and gene silencing in murine hepatocytes Article Snippet: The entry vector for inducible gene expression of YAP-Flag was generated by amplification from p2xFlag CMV2-YAP2 (Addgene #19045) using touchdown PCR followed by subcloning into pEN_TTmcs (Addgene #25755). .. The entry vector for inducible shRNA expression was generated using a validated shRNA sequence against mouse Hnf4α 22 that was modified in accordance with the pSLIK cloning protocol 19 followed by subcloning into Sequencing:Article Title: An in vivo transfection system for inducible gene expression and gene silencing in murine hepatocytes Article Snippet: The entry vector for inducible gene expression of YAP-Flag was generated by amplification from p2xFlag CMV2-YAP2 (Addgene #19045) using touchdown PCR followed by subcloning into pEN_TTmcs (Addgene #25755). .. The entry vector for inducible shRNA expression was generated using a validated shRNA sequence against mouse Hnf4α 22 that was modified in accordance with the pSLIK cloning protocol 19 followed by subcloning into Modification:Article Title: An in vivo transfection system for inducible gene expression and gene silencing in murine hepatocytes Article Snippet: The entry vector for inducible gene expression of YAP-Flag was generated by amplification from p2xFlag CMV2-YAP2 (Addgene #19045) using touchdown PCR followed by subcloning into pEN_TTmcs (Addgene #25755). .. The entry vector for inducible shRNA expression was generated using a validated shRNA sequence against mouse Hnf4α 22 that was modified in accordance with the pSLIK cloning protocol 19 followed by subcloning into Cloning:Article Title: An in vivo transfection system for inducible gene expression and gene silencing in murine hepatocytes Article Snippet: The entry vector for inducible gene expression of YAP-Flag was generated by amplification from p2xFlag CMV2-YAP2 (Addgene #19045) using touchdown PCR followed by subcloning into pEN_TTmcs (Addgene #25755). .. The entry vector for inducible shRNA expression was generated using a validated shRNA sequence against mouse Hnf4α 22 that was modified in accordance with the pSLIK cloning protocol 19 followed by subcloning into Subcloning:Article Title: An in vivo transfection system for inducible gene expression and gene silencing in murine hepatocytes Article Snippet: The entry vector for inducible gene expression of YAP-Flag was generated by amplification from p2xFlag CMV2-YAP2 (Addgene #19045) using touchdown PCR followed by subcloning into pEN_TTmcs (Addgene #25755). .. The entry vector for inducible shRNA expression was generated using a validated shRNA sequence against mouse Hnf4α 22 that was modified in accordance with the pSLIK cloning protocol 19 followed by subcloning into Polymerase Chain Reaction:Article Title: The Synthesis and Secretion of Versican Isoform V3 by Mammalian Cells: A Role for N-linked Glycosylation Article Snippet: .. Plasmid construction The open reading frame of rat V3 ( {"type":"entrez-nucleotide","attrs":{"text":"NM_001170559.1","term_id":"281604091","term_text":"NM_001170559.1"}} NM_001170559.1 ) was PCR-amplified with primers rV3SpeIF and rV3XbaIR ( ) introducing SpeI and XbaI restriction sites and a c-terminal Hag-tag and cloned into Article Title: Recombinant versican isoforms and related compositions and methods Article Snippet: .. Experimental Procedures Plasmid Construction The open reading frame of rat V3 (NM_001170559.1) was PCR-amplified with primers rV3SpeIF and rV3XbaIR (Table 3) introducing SpeI and XbaI restriction sites and a c-terminal hemagglutinin tag and cloned into Article Title: The Synthesis and Secretion of Versican Isoform V3 by Mammalian Cells: A Role for N-linked Glycosylation Article Snippet: .. The open reading frame of rat V3 ( {"type":"entrez-nucleotide","attrs":{"text":"NM_001170559.1","term_id":"281604091","term_text":"NM_001170559.1"}} NM_001170559.1 ) was PCR-amplified with primers rV3SpeIF and rV3XbaIR ( ) introducing SpeI and XbaI restriction sites and a c-terminal Hag-tag and cloned into Clone Assay:Article Title: The Synthesis and Secretion of Versican Isoform V3 by Mammalian Cells: A Role for N-linked Glycosylation Article Snippet: .. Plasmid construction The open reading frame of rat V3 ( {"type":"entrez-nucleotide","attrs":{"text":"NM_001170559.1","term_id":"281604091","term_text":"NM_001170559.1"}} NM_001170559.1 ) was PCR-amplified with primers rV3SpeIF and rV3XbaIR ( ) introducing SpeI and XbaI restriction sites and a c-terminal Hag-tag and cloned into Article Title: Recombinant versican isoforms and related compositions and methods Article Snippet: .. Experimental Procedures Plasmid Construction The open reading frame of rat V3 (NM_001170559.1) was PCR-amplified with primers rV3SpeIF and rV3XbaIR (Table 3) introducing SpeI and XbaI restriction sites and a c-terminal hemagglutinin tag and cloned into Article Title: An Amyotrophic Lateral Sclerosis-Associated Mutant of C21ORF2 Is Stabilized by NEK1-Mediated Hyperphosphorylation and the Inability to Bind FBXO3 Article Snippet: .. For generation of lentiviruses for DOX-inducible C21ORF2 constructs, cDNA encoding HA-C21ORF2 (WT or V58L) was cloned into Article Title: The Synthesis and Secretion of Versican Isoform V3 by Mammalian Cells: A Role for N-linked Glycosylation Article Snippet: .. The open reading frame of rat V3 ( {"type":"entrez-nucleotide","attrs":{"text":"NM_001170559.1","term_id":"281604091","term_text":"NM_001170559.1"}} NM_001170559.1 ) was PCR-amplified with primers rV3SpeIF and rV3XbaIR ( ) introducing SpeI and XbaI restriction sites and a c-terminal Hag-tag and cloned into Construct:Article Title: An Amyotrophic Lateral Sclerosis-Associated Mutant of C21ORF2 Is Stabilized by NEK1-Mediated Hyperphosphorylation and the Inability to Bind FBXO3 Article Snippet: .. For generation of lentiviruses for DOX-inducible C21ORF2 constructs, cDNA encoding HA-C21ORF2 (WT or V58L) was cloned into |